The new litter, all female, is believed to have been born in late July.

The new litter, all female, is believed to have been born in late July.

Known to researchers as P-109, P-110, P-111 and P-112, the new kittens were tagged and will be part of an ongoing 20-year-old study into how the big cats’ lives intertwine with the urban environment.

According to the National Park Service, the four cats each weighed about 4 pounds and appeared to be healthy.

To tag the four mountain lions on Aug. 24, a biologist used radio signals to track the mother and make sure she had left the den area.

Colleagues then took the kittens a short distance away and did a workup — which involves a physical exam, where body measurements are recorded, and attaching an ear tag — before the mother returned.

“Biologists remain in constant radio contact with each other,” the National Park Service said in a statement.

“Hence, there is little chance that they will encounter the mountain lion mother.”

“Hence, there is little chance that they will encounter the mountain lion mother.”

Researchers also added two more adult mountain lions to the study, a pair captured in the Santa Susana Mountains.